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CD4(+) T cell differentiation in infection: amendments to the Th1/Th2 axiom

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Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h

The appearance of mild-yellowish, mosaic symptoms was associated with a dramatic, 20-fold induction of defense-related genes like PR-1, chitinase and GST (Niehl et al., Potato virus Y (PVY) strains, the aggressive PVY NTN and the mild PVY N (Kogovsek et al., NTN -inoculated leaves displayed chlorotic and/or necrotic ringspot type lesions, while PVY N caused a mild chlorotic ringspot